The fusion of trapped ion mobility spectrometry (TIMS) with parallel reaction monitoring (PRM), particularly through the prm-PASEF ® technique, is indeed a groundbreaking development in proteomics. This innovative approach leverages the parallel accumulation-serial fragmentation (PASEF) mode to synchronize ion release from TIMS with selective precursor isolation, reducing the noise in the peptide ion spectra without sacrificing sensitivity.

Numerous software tools, including Spectronaut, have been developed to interpret the increasingly complex and voluminous raw data generated from mass-spectrometry-based proteomics data. These resulting datasets are typically extensive and challenging to analyze, necessitating substantial expertise in the field of proteomics.

This poster emphasizes the importance of MHC molecules in immune responses, particularly in cancer research, where comprehending tumor-associated antigens holds great significance. Accurately quantifying HLA class I/II neoantigens is essential for understanding how the immune system detects and reacts to cancerous cells. However, a significant limitation lies in the high input material requirement.

We devised an integrated immunopeptidomics (IMPX) workflow tailored for cell and tissue samples to address this challenge. This innovative approach allows for in-depth profiling of immunopeptides while substantially reducing the input material needed, which is particularly beneficial for PBMC samples.

Preclinical drug development involves in-vivo discovery studies whereby essential model organisms, including mouse and rat help to understand human biology and disease. Despite genetic similarities, distinctions in gene expression between humans and relevant model organisms underscore the challenge of translatability.

New: Spectronaut^® 19

Unlock Your Data's True Story

New: Spectronaut^® 19

Unlock Your Data's True Story

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